Isolation of Onchocerca lupi in Dogs and Black Flies, California, USA.

In southern California, ocular infections caused by Onchocerca lupi were diagnosed in 3 dogs (1 in 2006, 2 in 2012). The infectious agent was confirmed through morphologic analysis of fixed parasites in tissues and by PCR and sequencing of amplicons derived from 2 mitochondrially encoded genes and 1 nuclear-encoded gene. A nested PCR based on the sequence of the cytochrome oxidase subunit 1 gene of the parasite was developed and used to screen Simulium black flies collected from southern California for O. lupi DNA. Six (2.8%; 95% CI 0.6%-5.0%) of 213 black flies contained O. lupi DNA. Partial mitochondrial16S rRNA gene sequences from the infected flies matched sequences derived from black fly larvae cytotaxonomically identified as Simulium tribulatum. These data implicate S. tribulatum flies as a putative vector for O. lupi in southern California.

Effect of body position on intraocular pressure in dogs without glaucoma.

OBJECTIVE: To determine the effects of body position on intraocular pressure (IOP) in dogs without glaucoma. ANIMALS: 24 healthy dogs with no evidence of glaucoma. PROCEDURES: Dogs underwent ophthalmic examinations to ensure that no IOP-affecting ocular diseases were present. Each dog was sequentially placed in dorsal recumbency, sternal recumbency, and sitting position. For each of the 3 positions, IOP in the right eye was measured by use of an applanation tonometer immediately after positioning (0 minutes) and after 3 and 5 minutes had elapsed. The initial body position was randomly assigned; each position followed the other positions an equal number of times, and IOP measurements were initiated immediately after moving from one body position to the next. Proparacaine hydrochloride (0.5%) was applied to the right eye immediately prior to IOP measurements. RESULTS: Intraocular pressure was affected by body position. During the 5-minute examination, IOP decreased significantly in dogs that were dorsally recumbent or sitting but did not change significantly in dogs that were sternally recumbent. For the 3 positions, overall mean IOP differed significantly at each time point (0, 3, and 5 minutes). Mean IOP in dorsal recumbency was significantly higher than that in sternal recumbency at 0 and at 3 minutes; although the former was also higher than that in sitting position at 3 minutes, that difference was not significant. CONCLUSIONS AND CLINICAL RELEVANCE: Body position affects IOP in dogs. When IOP is measured in dogs, body position should be recorded and consistent among repeat evaluations.

Ophthalmic examination findings in adult pygmy goats (Capra hicus).

OBJECTIVE: To document normal ophthalmic findings and ocular abnormalities in captive adult pygmy goats. ANIMALS STUDIED: Ten healthy adult pygmy goats (five male, five female; 5-11 years of age; 26-45 kg body mass) underwent complete ophthalmic examinations. PROCEDURE: Direct illumination, diffuse and slit-beam biomicroscopy, indirect ophthalmoscopy, IOP measurements and Schirmer tear tests were performed. TonoVet rebound tonometry, followed by topical application of 0.5% ophthalmic proparacaine, and Tono-Pen XL applanation tonometry were performed in each eye to obtain estimates of IOP. RESULTS: Ophthalmic abnormalities included corneal scars and pigmentation, incipient cataracts, lenticular sclerosis, and vitreal veiling. Mean STT values were 15.8 mm/min, with a range of 10-30 mm/min. Mean IOP values were 11.8 mmHg for TonoVet-D, with a range of 9-14 mmHg; 7.9 mmHg for TonoVet-P, with a range of 6-12 mmHg; and 10.8 mmHg for Tono-Pen XL, with a range of 8-14 mmHg. CONCLUSIONS: Ophthalmic examination findings in adult pygmy goats, including normal means and ranges for STT and IOP measurements, using applanation and rebound tonometry, are provided.